Department of Medicine
Faculty Profiles by Division

Division of Endocrinology and Metabolism

Faculty Profiles

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photo Yusuke Sekine, PhD

Endocrinology and Metabolism

Assistant Professor of Medicine


Phone: 412-383-0698

Office: Bridgeside Point I - Room 567
100 Technology Dr.
Pittsburgh, PA 15219
Phone: 412-383-0698
Fax: 412-383-9055
Administrative Assistant:
Kathy Brickett
Address: 571.1 Bridgeside Point I
100 Technology Drive
Pittsburgh, PA 15219
Phone: 412-383-4416
Fax: 412-383-9055
Education and Training
BS, The University of Tokyo, 2005
MS, The University of Tokyo, 2007
PhD, The University of Tokyo, 2010
Postdoctoral Fellow, The University of Tokyo, 2010
Visiting Research Fellow, University of Cambridge, United Kingdom, 2013
Research Fellow, National Institutes of of Health, Bethesda, 2016
Research Interest
Dr. Sekine has been working on analyzing the molecular mechanisms underlying cellular responses to a variety of stresses including oxidative stress, endoplasmic reticulum stress, and metabolic stress. Using biochemical and cell genetic approaches, the Sekine Lab is currently focusing on acetyl-CoA fluctuation-dependent functional alterations in organelles (including nucleoli, mitochondria, and lysosomes) and activation of organelle-associated stress signaling pathways. A long-term goal is to understand the sensing mechanisms of metabolite fluctuations in cells and to reveal their relevance to human aging and age-associated diseases.
Selected Publications:
Liu, J., Zuo, Z., Sastalla, I., Liu, C., Jang, J.-Y., Sekine, Y., Li, Y., Pirooznia, M., Leppla, S. H., Finkel, T., Liu, S. Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors. Cell Host Microb. 2020; 28: 402-410.e5.
Baumer, Y., Dey, A. K., Gutierrez-Huerta, C. A., Khalil, N. O., Sekine, Y., Sanda, G. E., Zhuang, J., Saxena, A., Stempinski, E., Elnabawi, Y. A., Dagur, P. K., Ng, Q., Teague, H. L., Keel, A., Justin A., Rodante, J.A., Boisvert, W. A., Tsoi, L. C., Gudjonsson, J. E., et al. Hyperlipidaemia and IFNgamma/TNFalpha Synergism are Associated with Cholesterol Crystal Formation in Endothelial Cells Partly Through Modulation of Lysosomal pH and Cholesterol Homeostasis. EBioMedicine. 2020; 59: 102876.
Houston, R., Sekine, S., Calderon, M. J., Seifuddin, F., Wang, G., Kawagishi, H., Malide, D. M., Li, Y., Gucek, M., Pirooznia, M., Nelson, A. J., Stokes, M. P., Stewart-Ornstein, J., Mullett, S. J., Wendell, S. G., Watkins, S. C., Finkel, T., Sekine, Y. Acetylation-mediated remodeling of the nucleolus regulates cellular acetyl-CoA responses. PLoS Biol. 2020; 18: e3000981.
Zyryanova, A. F., Weis, F., Faille, A., Alard, A. A., Crespillo-Casado, A., Sekine, Y., Harding, H. P., Allen, F., Parts, L., Fromont, C., Fischer, P. M., Warren, A. J., Ron, D. Binding of ISRIB reveals a regulatory site in the nucleotide exchange factor eIF2B. Science. 2018; 359: 1533-1536.
Sekine, Y., Zyryanova, A., Crespillo-Casado, A., Amin-Wetzel, N., Harding, H. P., Ron, D. Paradoxical sensitivity to an integrated stress response blocking mutation in vanishing white matter cells. PLoS One. 2016; 11: e0166278.
Sekine, Y., Zyryanova, A., Crespillo-Casado, A., Fischer, P. M., Harding, H. P., Ron, D. Mutations in a translation initiation factor identify the target of a memory-enhancing compound. Science. 2015; 348: 1027-1030.
Halliday, M., Radford, H., Sekine, Y., Moreno, J., Verity, N., le Quesne, J., Ortori, C. A., Barrett, D. A., Fromont, C., Fischer, P. M., Harding, H. P., Ron, D., Mallucci, G. R. Partial restoration of protein synthesis rates by the small molecule ISRIB prevents neurodegeneration without pancreatic toxicity. Cell Death Dis. 2015; 6: e1672.
Mosallanejad, K., Sekine, Y., Ishikura-Kinoshita, S., Kumagai, K., Nagano, T., Matsuzawa, A., Takeda, K., Naguro, I., Ichijo, H. The DEAH-box RNA helicase DHX15 activates NF-kB and MAPK signaling downstream of MAVS during antiviral responses. Sci Signal. 2014; 7: ra40.
Sekine, Y., Hatanaka, R., Watanabe, T., Sono, N., Iemura, S., Natsume, T., Kuranaga, E., Miura, M., Takeda, K., Ichijo, H. The kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP5. Mol Cell. 2012; 48: 692-704.
Sekine, Y., Takagahara, S., Hatanaka, R., Watanabe, T., Oguchi, H., Noguchi, T., Naguro, I., Kobayashi, K., Tsunoda, M., Funatsu, T., Nomura, H., Toyoda, T., Matsuki, N., Kuranaga, E., Miura, M., Takeda, K., Ichijo, H. p38 MAP kinase regulates the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors. J Cell Sci. 2011; 124: 3006-3016.
Notable Achievements
UPMC Health System Competitive Medical Research Fund, 2020-2021
Samuel and Emma Winters Foundation, 2020-2021