Department of Medicine
Faculty Profiles by Division

Department of Medicine

Faculty Profiles

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photo Andrey A. Parkhitko, PhD

Endocrinology and Metabolism

Assistant Professor


Phone: 339-368-4594

Office: 569 Bridgeside Point I
100 Technology Drive
Pittsburgh, PA 15219
Phone: 339-368-4594
Fax: 412-383-9055
Administrative Assistant:
Kathy Brickett
Address: Bridgeside Point I, Room 571.1
100 Technology Drive
Pittsburgh, PA 15219
Phone: 412-383-4416
Education and Training
MS, Russian State Medical University, Russia; Partially Carried Out at Fox Chase Cancer Center, PA, 2007
PhD, Russian State Medical University, Russia; Partially Carried Out at Brigham & Women's Hospital, Harvard Medical School, Boston, MA, 2013
Postdoctoral Fellowship, Department of Genetics, Harvard Medical School, Boston, MA, 2020
Clinical Interest
Loss of metabolic homeostasis is a hallmark of aging. Using Drosophila as a model system, we demonstrated that aging is characterized by extensive reprogramming of methionine metabolism and delayed processing of SAH, a competitive inhibitor of a broad spectrum of methyltransferases. Moreover, we introduced into flies the bacterial enzyme Methioninase, which is capable of degrading methionine and has been safely tested in several cancer clinical trials. Methioninase dramatically extended fly lifespan. In addition, we demonstrated that tyrosine metabolism is altered with age due to the activation of the tyrosine degradation pathway. We found that suppression of mETC complex I drives upregulation of enzymes in the tyrosine degradation pathway and depletes tyrosine, which is required for the production tyrosine-derived neuromediators.
The immediate goals of our laboratory are to determine whether ‘resetting’ the methionine and tyrosine metabolic pathways back to normal is evolutionary conserved and can be translated to delay aging of higher organisms and improve phenotypes related to Alzheimer’s disease.

In the longer term, we will expand our studies by implementing carbon isotope-labeled nutrients coupled with mass spectrometry analysis to determine age-dependent changes in fates of nutrients at the whole-organism and tissue-specific levels; introduce bacterial enzymes similar to Methioninase that can potentially provide novel metabolic routes/functions to eukaryotic cells and help ‘reset’ age-dependent metabolic changes; and repurpose currently existing CRISPR/Cas9 multiplex systems for simultaneous targeting of different metabolic pathways to achieve additive/synergistic effects on lifespan.
For my complete bibliography, Click Here.
Selected Publications:
Parkhitko, A., Singh, A., Hsieh, S., Hu, Y., Binari, R., Lord, C.J., Hannenhalli, S., Ryan, C.J., Perrimon, N. Cross-species identification of PIP5K1C-, splicing- and ubiquitin-related pathways as potential targets for RB1-deficient cells. PLoS Genet. 2021; 17(2): e1009354.
Parkhitko, A.A., Ramesh, D., Wang, L., Leshchiner, D., Filine, E., Binari, R., Olsen, A.L., Asara, J.M., Cracan, V. Downregulation of the tyrosine degradation pathway extends Drosophila lifespan. Elife. 2020; 9: e58053.
Parkhitko, A., Filine, E., Mohr, S.E., Moskalev, A., Perrimon, N. Targeting metabolic pathways for extension of lifespan and healthspan across multiple species. Ageing Res Rev. 2020; 101188.
Parkhitko, A., Jouandin, P., Mohr, S.E., Perrimon, N. Methionine metabolism and methyltransferases in the regulation of aging and lifespan extension across species. Aging Cell. 2019; 18(6):e13034.
Rodriguez-Muela, N., Parkhitko, A., Grass, T., Gibbs, R.M., Norabuena, E.M., Perrimon, N., Singh, R., Rubin, L.L. Blocking p62-dependent SMN degradation ameliorates spinal muscular atrophy disease phenotypes. J Clin Invest. 2018; pii:95231.
Parkhitko, A.A., Binari, R., Zhang, N., Asara, J.M., Demontis, F., Perrimon, N. Tissue-specific down-regulation of S-adenosyl-homocysteine via suppression of dAhcyL1/dAhcyL2 extends health span and life span in Drosophila. Genes Dev. 2016; 30(12): 1409-22.
Parkhitko, A.A., Priolo, C., Coloff, J.L., Yun, J., Wu, J.J., Mizumura, K., Xu, W., Malinowska, I., Yu, J., Kwiatkowski, D.J., Locasale, J.W., Asara, J.M., Choi, A., Finkel, T., Henske, E.P. Autophagy-dependent Metabolic Reprogramming Leads to Pentose Phosphate Pathway Addiction in Cells with Hyperactive mTORC1. Mol Cancer Res. 2014; 12(1): 48-57.
Li, C., Lee, P.S., Sun, Y., Gu, X., Zhang, E., Guo, Y., Wu, C.L., Auricchio, N., Priolo, C., Li, J., Csibi, A., Parkhitko, A., Morrison, T., Planaguma, A., Kazani, S., Israel, E., Xu, K.F., Henske, E.P., Blenis, J., Levy, B.D., Kwiatkowski, D., Yu, J.J. Estradiol and mTORC2 cooperate to enhance prostaglandin biosynthesis and tumorigenesis in TSC2-deficient LAM cells. J Exp Med. 2014; 211(1): 15-28.
Csibi, A., Fendt, S.M., Li, C., Poulogiannis, G., Choo, A.Y., Chapski, D.J., Jeong, S.M., Dempsey, J.M., Parkhitko, A., Morrison, T., Henske, E.P., Haigis, M.C., Cantley, L.C., Stephanopoulos, G., Yu, J., Blenis, J. The mTORC1 Pathway Stimulates Glutamine Metabolism and Cell Proliferation by Repressing SIRT4. Cell. 2013; 153(4): 840-54.
Parkhitko, A.A., Myachina, F., Morrison, T., Hindi, K., Auricchio, N., Karbowniczek, M., Wu, J., Finkel, T., Kwiatkowski, D., Yu, J., Henske, E.P. Tumorigenesis in Tuberous Sclerosis Complex is Autophagy and p62/Sequestosome 1-dependent. Proc Natl Acad Sci USA. 2011; 108(30): 12455-60.
Notable Achievements
Charles A. King Foundation Award, 2017
NIH Pathway to Independence K99/R00 Award, 2018